Label-free detection with polydiacetylene vesicle immobilized on the substrate

Polyacetylene vesicle has attracted attentions due to its unique chromatic properties, such as blue-to-red transition. The liposome-like vesicle was immobilized on solid substrates to detect small quantity analytes. In this case, fluorescence was used for detection. We successfully detected protein and bacteria using antibodies or aptamers on the vesicles as probes. Recently, we developed a different immobilization method, which provided much better immobilization efficiency. Target nucleic acid was successfully detected by hybridization to its complementary DNA probe on PDA vesicle. We introduced a stable label-free optical sensor using PDA liposome.